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. 2024 Mar 6;51(5):63. doi: 10.3892/or.2024.8722

Figure 3.

Figure 3.

Total, cytosolic and nuclear expression of MITF, PIAS4 and VHL in control cells and PDCs. Western blotting showing the (A) total (B) cytoplasmic and (C) nuclear protein ex pression of PIAS4, MITF and VHL in Hct116 and LoVo control cells and PDCs. (D) Statistical analysis of the expression differences of (a) total, (b) cytoplasmic and (c) nuclear MITF expression; (d) total, (e) cytoplasmic and (f) nuclear VHL expression; (g) total, (h) cytoplasmic and (i) nuclear PIAS4 expression in Hct116 and LoVo control and PDCs. (E) Immunocytochemical staining of PIAS4, MITF and VHL in Hct116 and LoVo control cells and PDCs. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. PIAS4, protein inhibitor of activated STAT protein 4; MITF, microphthalmia-associated transcription factor; VHL, von Hippel-Lindau disease tumor suppressor PDCs, daughter cells; Tre, polyploid giant cells PDCs.