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. 2024 Mar 6;51(5):63. doi: 10.3892/or.2024.8722

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Copyright: © 2024 Zheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — The mutation of HIF1α at different lysine sites is associated with the proliferation, migration and invasion abilities of PDCs. (A) Colony formation of 50, 100 and 150 (a) Hct116 and (b) LoVo PDCs before and after transfection with K391R and K477R double mutants. (B) Wound healing assay of (a) Hct116 and (b) LoVo PDCs before and after transfection with K391R and K477R double mutants at 0 h and 24 h (magnification, ×100). (C) Invasion and migration abilities of (a) Hct116 and (b) LoVo PDCs before and after transfection with K391R and K477R double mutants. (D) (a) Differences in colony formation efficiency and (b) statistical analysis of wound healing index in Hct116 and LoVo PDCs before and after transfection with K391R and K477R double mutants. (E) Comparison of the average cell number in invasion and migration assay of (a) Hct116 and (b) LoVo PDCs before and after transfection with K391R and K477R double mutants. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 ns, no significance. PDCs, daughter cells; PGCCs, polyploid giant cells; Tre, PGCCs with PDCs.