Figure 1.
Effect of α-PA combined with 5-FU treatment on HT-29 cell viability and apoptosis. HT-29 cells (1.0×105 cells/30-mm plate) were treated with 50, 100 or 250 µg/mM α-PA combined with 5-FU for 72 h. (A) Viability of HT-29 cells. (B) Morphological examination of HT-29 cells (×400 magnification). (C) Immunocytochemical staining with Annexin V (green) and PI (red) (×400 magnification). Quantitative analysis of the relative intensities of (D) Annexin V and (E) PI staining after treatment. Untreated cells and cells treated with 5 µM 5-FU or 250 mM α-PA for 72 h served as control groups. Data are presented as the mean ± SD (n=3-5). *P<0.05 vs. Control; #P<0.05 vs. 5-FU. 5-FU, 5-fluorouracil; α-PA, α-phellandrene.