Figure 2.

Effect of α-PA combined with 5-FU treatment on p53, Bax and Bcl-2 protein expression, and MMP levels in HT-29 cells. HT-29 cells (1.0×10⁵ cells/30-mm plate) were treated with 50, 100 or 250 µg/mM α-PA combined with 5-FU for 72 h. Western blotting was performed to determine the expression levels of (A) p53, (B) Bax and (C) Bcl-2 in HT-29 cells after treatment. (D) Quantitative analysis of (E) immunocytochemical staining with DiOC6 (green) and Hoechst 33342 (blue) to analyze the relative MMP levels (×400 magnification). Data are presented as the mean ± SD (n=3-5). *P<0.05 vs. Control; ^#P<0.05 vs. 5-FU. 5-FU, 5-fluorouracil; α-PA, α-phellandrene. 5-FU, 5-fluorouracil; α-PA, α-phellandrene; MMP, mitochondrial membrane potential.