Figure 4.

Effect of α-PA combined with 5-FU treatment on cytochrome c and caspase mRNA expression in HT-29 cells. HT-29 cells (1.0×10⁵ cells/30-mm plate) were treated with 50, 100 or 250 µg/mM α-PA combined with 5-FU for 72 h. Reverse transcription-quantitative polymerase chain reaction analysis of the mRNA expression levels of (A) caspase-8, (B) Bid, (C) cytochrome c, (D) caspase-9 and (E) caspase-3. T. Data are presented as the mean ± SD (n=3-5). *P<0.05 vs. Control; ^#P<0.05 vs. 5-FU. 5-FU, 5-fluorouracil; α-PA, α-phellandrene. 5-FU, 5-fluorouracil; α-PA, α-phellandrene.