Reviewer name and names of any other individual's who aided in reviewer	Qing Liu
Do you understand and agree to our policy of having open and named reviews, and having your review included with the published papers. (If no, please inform the editor that you cannot review this manuscript.)	Yes
Is the language of sufficient quality?	Yes
Please add additional comments on language quality to clarify if needed	No
Are all data available and do they match the descriptions in the paper?	Yes
Additional Comments
Are the data and metadata consistent with relevant minimum information or reporting standards? See GigaDB checklists for examples <a href="http://gigadb.org/site/guide" target="_blank">http://gigadb.org/site/guide</a>	Yes
Additional Comments
Is the data acquisition clear, complete and methodologically sound?	Yes
Additional Comments
Is there sufficient detail in the methods and data-processing steps to allow reproduction?	Yes
Additional Comments
Is there sufficient data validation and statistical analyses of data quality?	Yes
Additional Comments
Is the validation suitable for this type of data?	Yes
Additional Comments
Is there sufficient information for others to reuse this dataset or integrate it with other data?	Yes
Additional Comments
Any Additional Overall Comments to the Author	This updated double haploid perennial ryegrass (Lolium perenne L.) showed contig N50 of 120 Mb, total BUSCO score=99%, which verified that the improved assembly can serve a reference for Lolium species using 50-fold coverage Hi-C data. The article is well edited except for below revision points. The minor revision is suggested for the current version. 1 Please elucidate the Kyuss v2.0, whether its reference is the same as Kyuss v1.0, if same or separate reference please elucidate. 2 In Table 3 of page 6, What the repeat element number for each family, could authors listed in number and proportion in order to clear the family category, for example, is the number of rolling-circles the same for Heltrons? 3 Tandem repeat or satellite or centromere location data, could author provide for the updated assembly of the Lolium species. 4 For Figure 1, what the heterozygosity and k-mer estimated genome size, I can’t find the data. 5 In Figure 3A, lowercase letter a, b, c , d and e are suggested to subsittute the A, B, C, D and E in order to avoid Figure 3A and Figure 3AA
Recommendation	Minor Revision