Fig. 8.

PC/Fe@c(RGDfc) coating promoted cell osteogenesis in vitro. Representative images of ALP staining of mBMSCs cultured on different coatings on days 3 and 7 of cell culture (a) and detection of ALP activity (b). Representative images of ARS staining (c) and quantification of calcium nodules (d) of mBMSCs cultured on different substrates for 14 and 21 days. LCSM images of mBMSCs cultured on different coatings for 7 d, and nuclei (blue) and collagen I(red) staining were performed. N = 3, no significance noted as "ns", ***p < 0.001 using t-test. N = 3, no significance noted as "ns," *p < 0.05, **p < 0.01, ***p < 0.001, and ^#p < 0.05, ^##p < 0.01 or ^###p < 0.001 compared with the Control group, using t-test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)