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. 2024 Mar 8;36:301–316. doi: 10.1016/j.bioactmat.2024.02.031

Fig. 2.

Fig. 2

Differentiation of hematopoietic and lymphoid progenitors from human iPSCs. (A) (i) phase-contrast images captured on Day 3, Day 6, and Day 8 show the progression of myeloid progenitors, while (ii) qualitative analysis of CD34 and CD43 expression is conducted through immunocytochemistry (ICC), and (iii) flow cytometry provides a quantitative assessment of co-staining of CD34 and CD43. (B) (i) phase-contrast images tracking the development of lymphoid progenitors on Day 15, Day 20, Day 25, Day 30, and Day 35. Additionally, (ii) immunocytochemistry detects the expression of ZBTB16 and CD25 markers on Day 35, and (iii) flow cytometry quantifies ZBTB16, CD25, and CD7 on the same day of differentiation. ZBTB-16, Zinc finger and BTB domain containing 16; CD, Cluster of differentiation; Hoe, Hoechst 33,342. The scale bars in (A) and (B) represent 100 μm, corresponding to the magnification of 20x for ICC images and 10x for the phase contrast images.