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Table 3. Dissociation ACSF—Additional reagents
| Reagent | Final concentration | Stock concentration | Amount per 100 mL |
|---|---|---|---|
| HEPES (pH 7.4 at 21°C) | 20 mM | 1 M | 2 mL |
| Phenol red | 0.001% | 0.5% | 200 μL |
| Trehalose dihydrate | 132 mM | Solid | 5 g |
Store finished Dissociation ACSF at 4°C for up to 1 week.
