KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-FLAG (M2) | Sigma Aldrich | Cat. #: F1804; RRID:AB_262044 |
| Anti-DDDDK-HRP | abcam | Cat. #: ab1238; RRID:AB_299061 |
| Anti-HSP90 (F-8) | Santa Cruz | Cat. #: sc-13119; RRID:AB_675659 |
| Anti-HSP70/HSC70 (N27F3–4) | StressMarq Biosciences | Cat. #: SMC-104B; RRID:AB_2820319 |
| EZview Red ANTI-FLAG agarose beads | Sigma Aldrich | Cat. #: F2426; RRID:AB_2616449 |
| Chemicals, peptides, and recombinant proteins | ||
| MDYKDHDGDYKDHDIDYKDDDDK (3x-FLAG) | Biomatik | Cat. #: 56305, custom peptide synthesis |
| LR Clonase II Enzyme Mix | Invitrogen | Cat. #: 11791100 |
| BP Clonase II Enzyme Mix | Invitrogen | Cat. #: 11789020 |
| Phusion High-Fidelity DNA Polymerase | New England Biolabs | Cat. #: M0530L |
| Critical commercial assays | ||
| Renilla Luciferase Assay System | Promega | Cat. #: E2820 |
| Nano-Glo Luciferase Assay System | Promega | Cat. #: N1120 |
| SuperSignal ELISA Pico Chemiluminescent Substrate | Thermo Scientific | Cat. #: 37069 |
| PureYield Plasmid Miniprep System | Promega | Cat. #: A1222 |
| NucleoSpin Gel and PCR Clean-Up | Takara Bio | Cat. #: 740609.250 |
| Immobilon Western Chemiluminescent HRP Substrate | Millipore Sigma | Cat. #: WBKLS0500 |
| Deposited data | ||
| Mutant chaperone interaction and computational data | This study | https://data.mendeley.com/datasets/rmj7fwm6n7/1 |
| Experimental models: Cell lines | ||
| Rluc-HSPA8 | Taipale et al.,41 2012 | R156 |
| Rluc-HSP90AB1 | Taipale et al.,41 2012 | R166 |
| Nluc-HSPA8 | This paper | N156 |
| Nluc-HSP90AB1 | This paper | N166 |
| Recombinant DNA | ||
| pDONR221–1314-BRCA1-STOP | This paper | pKlab182 |
| pDONR221-ATG-1528-BRCA1 | This paper | pKlab183 |
| pDONR221-ATG-1314-BRCA1 | This paper | pKlab184 |
| pcDNA3.1–1314-BRCA1-WT-V5–3xFLAG | This paper | pKlab187 |
| pcDNA3.1–1314-BRCA1–V5–3xFLAG variant library (for details see Table S1) | This paper | BRCT variant library |