Figure 2.

Methods for controlling axon guidance. Schematic representations of approaches to control axon guidance via physical (top) and chemical (bottom) patterning. Detailed explanations of each method can be found in the respective subsection [engineered substrates; microfluidics; microcontact printing (µCP); in situ techniques]. For each method, the nature of the guiding feature (continuous; discontinuous, or custom) and possible direction for axon growth (unidirectional; bidirectional; multidirectional, or custom) are described.