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. 2021 Feb 21;28(6):e16339. doi: 10.1111/jcmm.16339

Figure 2.

Figure 2

Decreased expression of GLP‐1R in islets from FXR−/− mice. A, B, qPCR (A) and Western blot (B) analysis of GLP‐1R mRNA (A) and protein (B) expression in scramble control and shFXR INS‐1 832/13 cells. Bars represent means ± SEM, n = 4 (A) or 3 (B). *P < 0.05. C, D, The expression of GLP‐1R mRNA (C) and protein (D) was determined in islets from FXR+/+ and FXR−/− mice. Bars represent means ± SEM, n = 5 (C) or 4 (D). **P < 0.01. E, F, cAMP level (E) and insulin secretion (F) were assayed in scramble and shFXR INS‐1 832/13 cells. The cells were stimulated with 16.8 mmol/L glucose in the absence or presence of 10 nmol/L GLP‐1 for 30 min. Data represent means ± SEM, n = 4. **P < 0.01. G, H, cAMP level (G) and insulin secretion (H) were determined in islets from FXR+/+ and FXR−/− mice. Bars represent means ± SEM, n = 4 (G) or 9 (H). *P < 0.05; **P < 0.01