Figure 3.

FXR activation promotes FXR binding to the promoter of GLP‐1R. A, Alignment of putative FXR binding element (FXRE) at the Glp‐1r promoter in various species. B, C, The occupancies of FXR at the Glp‐1r promoter were determined in INS‐1 832/13 cells treated with 5 μmol/L GW4064 for 2 h (B) or transfected with either scramble or shFXR (C). Control IgG was used as a negative control. *P < 0.05; **P < 0.01. D, Mutation of FXRE at the Glp‐1r promoter. E, Effect of GW4064 on the Glp‐1r promoter activity. Bars represent means ± SEM, n = 6. **P < 0.01