Figure 4.

FXR mediates histone 3 acetylation, SRC1 binding to the promoter of Glp‐1r A, C, ChIP assays were employed to determine the histone H3 acetylation (A) and SRC1 binding (C) at the Glp‐1r promoter in INS‐1 832/13 cells stimulated with DMSO or 5 μmol/L GW4064 for 2 h. IgG was used as a negative control. Bars represent means ± SEM of 4 (A) or 3 (B) independent experiments. *P < 0.05. B, D, As in (A, C), but ChIP assays were performed in scramble or shFXR INS‐1 832/13 cells. Bars represent means ± SEM, n = 3. **P < 0.01. E, GLP‐1R protein expression was determined in scramble or shSRC1 INS‐1 832/13 cells stimulated with DMSO or 5 μmol/L GW4064 for 48 h. Bars represent means ± SEM, n = 3. *P < 0.05