Table 2. Direct infusion mass spectrometry serum acylcarnitines (μM) from 14 lean (L-MAINT) and 16 obese cats during weight maintenance (O-MAINT) and after 10-week energy restriction (O-RESTRICT).
| Metabolite (μM) | L-MAINT (n = 14) | O-MAINT (n = 16) | O-RESTRICT (n = 16) | P-Value | |||
|---|---|---|---|---|---|---|---|
| L-MAINT: O-MAINT | L-MAINT: O-RESTRICT | O-MAINT: O-RESTRICT | |||||
| Free | CO | 25.80 (13.20–50.20) | 28.90 (19.60–53.50) | 29.00 (17.70–34.30) | 0.40 | 0.69 | 0.62 |
| Short | C2 | 2.38 (1.64–6.83) | 2.84 (1.63–4.26) | 3.33 (2.26–6.30) | 0.62 | 0.06 | 0.06 |
| C3 | 0.15 ± 0.05 | 0.17 ± 0.06 | 0.16 ± 0.04 | 0.21 | 0.48 | 0.26 | |
| C4 | 0.14 ± 0.03 | 0.15 ± 0.03 | 0.16 ± 0.04 | 0.47 | 0.21 | 0.43 | |
| C5 | 0.18 ± 0.06 | 0.19 ± 0.04 | 0.19 ± 0.05 | 0.50 | 0.60 | 0.86 | |
| C5:1 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 | 0.29 | 0.06 | 0.21 | |
| Medium | C12 | 0.07 ± 0.004 | 0.07 ± 0.005 | 0.07 ± 0.003 | 0.73 | 0.67 | 0.98 |
| Long | C14 | 0.11 (0.08–0.16) | 0.11 (0.080–0.19) | 0.12 (0.090–0.19) | 0.66 | 0.38 | 0.38 |
| C14:1 | 0.16 ± 0.01 A | 0.16 ± 0.01 A | 0.21 ± 0.02 B | 0.95 | 0.04 | 0.01 | |
| C14:1 OH | 0.030 ± 0.002AB | 0.029 ± 0.001 A | 0.034 ± 0.002 B | 0.73 | 0.21 | 0.01 | |
| C14:2 | 0.024 (0.01–0.06) A | 0.024 (0.01–0.03) A | 0.033 (0.02–0.07) B | 0.97 | 0.04 | 0.006 | |
| C14:2 OH | 0.021 ± 0.001 | 0.020 ± 0.001 | 0.020 ± 0.001 | 0.63 | 0.83 | 0.61 | |
| C16 | 0.19 (0.10–0.30)AB | 0.18 (0.14–0.31) A | 0.24 (0.15–0.34) B | 0.91 | 0.12 | 0.02 | |
| C16:1 | 0.073 (0.056–0.12) A | 0.070 (0.048–0.15) A | 0.10 (0.047–0.17) B | 0.82 | 0.01 | 0.007 | |
| C16:1 OH | 0.019 ± 0.001 A | 0.021 ± 0.001AB | 0.024 ± 0.001 B | 0.29 | 0.01 | 0.07 | |
| C16:2 | 0.014 (0.008–0.039) | 0.016 (0.010–0.023) | 0.02 (0.0090–0.037) | 0.51 | 0.21 | 0.21 | |
| C18 | 0.098 (0.059–0.16)AB | 0.096 (0.059–0.15) A | 0.13 (0.078–0.17) B | 0.63 | 0.16 | 0.03 | |
| C18:1 | 0.18 (0.095–0.34) A | 0.19 (0.10–0.48) A | 0.25 (0.12–0.46) B | 0.47 | 0.02 | 0.01 | |
| C18:1 OH | 0.022 ± 0.001 A | 0.023 ± 0.001 A | 0.026 ± 0.002 B | 0.66 | 0.05 | 0.03 | |
| C18:2 | 0.070 (0.05–0.16)AB | 0.076 (0.04–0.16) A | 0.11 (0.04–0.18) B | 0.73 | 0.10 | 0.02 | |
| Total | Short chain Acylcarnitines | 2.87 (2.06–7.63) A | 3.46 (1.97–4.82) A | 3.81 (2.76–6.81) B | 0.34 | 0.049 | 0.02 |
| Long chain Acylcarnitines | 1.05 ± 0.08 A | 1.09 ± 0.07 A | 1.32 ± 0.09 B | 0.75 | 0.03 | 0.04 | |
| Acylcarnitines | 32.36 ± 2.92 | 35.36 ± 2.21 | 33.46 ± 1.64 | 0.41 | 0.74 | 0.82 | |
Normally distributed data expressed as mean ± SE
Non-parametric data expressed as median (min-max).
Superscript capital letters (A, B) denote significant differences between groups with different letters where a p-value <0.05 is considered significant
No superscript letters in a row indicates no significant differences between groups for the measured parameter
Short-chain Acylcarnitines = C2+C3+C4+C5+C5:1
Long-chain Acylcarnitines = C14+C14:1+C14:1OH+C14:2+C14:2OH+C16+C16:1 = C16:1OH+C16:2+C18+C18:1+C18:1OH+C18:2