Table 8. Direct infusion mass spectrometry serum sphingolipids (μM) from 14 lean (L-MAINT) and 16 obese (O-MAINT) cats during weight maintenance and during 10-week energy restriction (O-RESTRICT).

Metabolite (μM)	L-MAINT (n = 14)	O-MAINT (n = 16)	O-RESTRICT (n = 16)	P-Value
				L-MAINT: O-MAINT	L-MAINT: O-RESTRICT	O-MAINT: O-RESTRICT
Hydroxysphingomyeline C14:1	11.44 ± 0.56	11.53 ± 0.84	11.91 ± 0.56	0.93	0.55	0.52
Hydroxysphingomyeline C16:1	2.97 ± 0.17AB	2.73 ± 0.20 A	3.14 ± 0.15 B	0.37	0.46	0.007
Hydroxysphingomyeline C22:1	15.82 ± 0.88	14.39 ± 1.03	14.86 ± 0.59	0.31	0.36	0.57
Hydroxysphingomyeline C22:2	6.49 ± 0.29	6.02 ± 0.51	6.56 ± 0.38	0.42	0.89	0.16
Hydroxysphingomyeline C24:1	1.85 ± 0.08	1.85 ± 0.13	2.07 ± 0.10	0.99	0.10	0.08
Sphingomyeline C16:0	113.50 (66.50–144.00)	99.75 (64.60–157.00)	108.00 (79.90–143.00)	0.95	0.69	0.51
Sphingomyeline C16:1	5.95 ± 0.29AB	6.10 ± 0.39 A	6.89 ± 0.37 B	0.75	0.06	0.01
Sphingomyeline C18:0	17.54 ± 0.97AB	15.88 ± 1.12 A	18.86 ± 0.86 B	0.28	0.32	0.03
Sphingomyeline C18:1	3.62 ± 0.23AB	3.37 ± 0.27 A	4.22 ± 0.25 B	0.50	0.10	0.0007
Sphingomyeline C20:2	0.42 (0.25–0.60)	0.42 (0.23–0.74)	0.42 (0.24–0.56)	0.56	0.96	0.66
Sphingomyeline C24:0	20.10 (12.40–24.60)	17.80 (14.40–28.50)	17.50 (14.40–22.80)	0.76	0.23	0.43
Sphingomyeline C24:1	25.95 ± 1.43	25.40 ± 1.44	27.19 ± 1.04	0.79	0.48	0.20
Sphingomyeline C26:0	0.35 ± 0.03	0.36 ± 0.02	0.36 ± 0.02	0.25	0.90	0.05
Sphingomyeline C26:1	0.31 ± 0.03AB	0.26 ± 0.03 A	0.34 ± 0.02 B	0.36	0.41	0.03
Total Sphingolipids	228.90 (145.57–289.07)	196.52 (137.04–310.68)	224.43 (165.24–291.62)	0.74	0.70	0.35

Normally distributed data expressed as mean ± SE. Non-parametric data expressed as median (min-max).

Superscript capital letters (A, B) denote significant differences between groups with different letters where a p-value <0.05 is considered significant

No superscript letters in a row indicates no significant differences between groups for the measured parameter