FIG. 2.

EnvA expression in MLV(Tva) target cells. Equivalent amounts of protein from cell lysates from recombinant RSV-A chronically infected QT6 cells (QT6 AP), 293T cells transiently transfected with pCB6 EnvA (293T EnvA), and NIH 3T3 cells stably expressing pCB6 EnvA (3T3EnvA) were resolved by SDS-PAGE (10% polyacrylamide), transferred to nitrocellulose, and then analyzed by Western blotting with α-AMV polyclonal sera. (A) Short exposure. (B) Overexposure to better visualize EnvA expression in QT6 AP cells. Molecular size standards (in kilodaltons) are indicated on the left.