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. 2024 Mar 15;10(11):eadk7160. doi: 10.1126/sciadv.adk7160

Fig. 1. MYOCD nuclear condensates are associated with components of active transcription.

Fig. 1.

(A) Immunofluorescence (IF) imaging of Myc-tagged MYOCD ectopically expressed in COS-7 cells with Myc antibody targeting the epitope. Scale bar, 5 μm. (B) Line profile plot presented as arbitrary fluorescence units (AFU)/background across the 3-μm white line shown in (A). (C) IF imaging of Flag-tagged SRF ectopically expressed in COS-7 cells with Flag antibody targeting the epitope. Scale bar, 5 μm. (D) Line profile plot presented as AFU/background across the 3-μm white line shown in (C). (E) IF imaging of Myc-tagged MYOCD and other transcription factors (Flag-tagged SRF and Flag-tagged NRF1) coexpressed in COS-7 cells. Scale bar, 5 μm. (F) Line profile plot presented as AFU/background for either MYOCD (green) or the indicated coexpressed transcription factor (red) across the 3-μm white line shown in (E). (G) Representative micrographs of COS-7 cells expressing MYOCD-mEGFP (green) and co-IF for factors, including H3K27ac, RPB1, HP1α, and NPM1 (red). Scale bar, 5 μm.