FIG. 5.

Analysis of the EBNA2 responsiveness of wild-type (wt) and CBF2 Cp mutants. (A) Results of single-dose transfections to compare levels of EBNA2 transactivation of reporter plasmids containing eight tandem copies of the EBNA2 enhancer. DG75 cells were cotransfected with 2 μg of the target plasmid and 0.5 μg of the effector EBNA2-expressing plasmid. Results are averages from two independent experiments. mut., mutant. (B) Dose responses for comparison of levels of EBNA2 transactivation of reporter plasmids containing the Cp −1021-to-+3 sequence. Cells were transfected with 2 μg of target plasmid and 0.2, 0.4, 0.8, and 1.6 μg of effector EBNA2-expressing plasmid. Results are averages from five independent experiments. Standard errors of the means are indicated with T bars.