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[Preprint]. 2024 Mar 6:2024.03.06.583756. [Version 1] doi: 10.1101/2024.03.06.583756

Figure 3. CD47 is required for cell colony expansion and proliferation.

Figure 3.

Marrow and periosteal MSC harvested from the femur and tibia of WT and CD47-null mice. a, CFU-F of WT (n = 8) and CD47-null (n = 12) marrow-derived cells. b, Representative CFU-f plate from WT (left) and CD47-null (right) mice. c, MTT assay of marrow-derived cells MSC from WT (n= 4) and CD47-null (n=4) mice at 1, 6, 10, and 14 days in culture. d, MTT assay of marrow MSC from WT (n= 6) and CD47-null (n=6) after 1 passage at 3, 6, and 9 days in culture. e, CCK8 assay of periosteal MSC from WT (n= 8) and CD47-null (n=8) after 1 passage at 3, 5, 7, and 9 days in culture. f, CFU-F of periosteal MSC from WT (n= 6) and CD47-null (n=6) mice. Mean ±SD, *P<0.05, **P<0.01, ***P<0.001 two-sided t tests.