Figure 7. Genetic knockout of CD47 limits recovery of whole limb perfusion, but shows local increases in endothelial cells after induced ischemia.

Relative perfusion WT and CD47-null mice at days 0–9 post-ischemic surgery using laser doppler flowmetry. a, Perfusion data was fit to a one-phase non-linear curve for WT (fit is the solid blue line with 95% CI in transparent blue; R²=0.7609) and CD47-null (fit is the solid red line with 95% CI in transparent red; R²=0.6223). The rate of recovery was faster, but maximum recovery was significantly lower in CD47-null compared to WT mice (P<0.0001, extra sum-of-squares F test). b, Representative immunofluorescence staining of CD31 and EMCN in CD47-null (n=7) and WT (n=8) ischemic fractures at 20X (Scale bar = 100 µm). c, CD31 quantification in WT and CD47-null ischemic fracture calluses at day 4 post-fracture at peripheral and central regions of the fracture callus. D, EMCN quantification in WT and CD47-null ischemic fracture calluses at day 4 post-fracture at peripheral and central regions. Mean ±SD, *P<0.05, **P<0.01, two-sided t test.