Figure 3: Antigen activated cells express markers of Th17 and Th1 cells upon stimulation with RVMA and classical antigens respectively.
Cryopreserved PBMCs from participants in cohort 1 were stimulated with antigens for a total of 20 hours in the presence of costimulatory antibodies anti-CD28 and anti-CD49d and anti-CD40 blocking antibody, and antigen activated cells identified by CD154 surface expression on CD4+ T cells. (A) Representative flow cytometry plots. (B) CD154 surface expression on CD4+ T cells, CD154+ used to identify antigen activated T cells. (C) Expression of helper T cell lineage defining transcription factors (RORĪ³T = Th17) and (T-bet = Th1) and (D) Expression of chemokine receptors (CCR6 = Th17 marker ) and (CXCR3 = Th1 marker) on antigen activated T cells. Statistics: Wilcoxon matched pairs test.