Skip to main content
[Preprint]. 2024 Jul 17:2024.03.07.583498. Originally published 2024 Mar 9. [Version 4] doi: 10.1101/2024.03.07.583498

Figure 2.

Figure 2.

Electron capture charge reduction of GroEL. The top panel shows the mass spectrum of GroEL with the typical charge distribution generated by spraying from ammonium acetate (i.e., no gas phase charge reduction). Panels B-F show results from increases in electron capture charge reduction, with the device voltage that initiates ECCR increasing in two-volt steps (in-source trapping of −75V and trap gas setting of 7). Panel G is maximum ECCR obtained for this sample (see Table S1). Panel H is from a different sample from A-G and shows more extensive charge reduction with peaks up to 107K m/z; more 15-mer of GroEL (additional, lower abundance peaks) was present in this sample, an older sample that had aged after refolding (in-source trapping of −100 V and trap gas setting of 8). Panel I is a zoom in on the high m/z range of panel H.