Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Recombinant Anti-Fibronectin antibody (Rabbit monoclonal) |
abcam | ab268020 | IF (1:50) |
| Antibody | Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 594 (Goat polyclonal) |
Invitrogen | A32740 | IF (1:100) |
| Peptide, recombinant protein | Animal-Free Recombinant Human FGF-basic (bFGF) | PeproTech | AF10018B | |
| Peptide, recombinant protein | VEGF Recombinant Human Protein | Life Technologies | PHC9394 | |
| Chemical compound, drug | Sphingosine-1-phosphate | Sigma-Aldrich | S9666 | |
| Chemical compound, drug | Phorbol myristate acetate | Sigma-Aldrich | P1585 | |
| Biological sample (Homo sapiens) | Primary human brain microvascular endothelial cells transfected with GFP-expressing lentivirial particles. | Angio-Proteomie | cAP-0002GFP | Isolated from normal human brain tissue |
| Software, algorithm | IMARIS 9.8.0 | Bitplane | https://imaris.oxinst.com | |
| Software, algorithm | PRISM 9.0.0 | GraphPad | https://www.graphpad.com/features | |
| Software, algorithm | Jupyter Notebook 6.1.6 | Project Jupyter | https://jupyter.org/install | |
| Software, algorithm | Python 3.12.1 | Python Software Foundation | https://www.python.org | |
| Other | Hoechst 33342 | Thermo Fisher | H3570 | IF (5 µg/ml) |