Table 1. Experimental affinities of different SpCAF-1 constructs with a 40 bp dsDNA measured by MicroScale thermophoresis (MST) fitted with the Hill model (Tso et al., 2018).

Construct	EC50 (µM)	Hill coeff., h
Pcf1_KER	1.1±0.2	3.3±0.5
Pcf1_KER*	12.2±0.7	1.5±0.3
Pcf1_KER-PIP	1.9±0.3	5.2±0.9
Pcf1_WHD	Not detected	Not detected
SpCAF-1 WT	0.7±0.1	2.7±0.2
SpCAF-1-ΔWHD	0.7±0.1	2.3±0.3
SpCAF-1-KER*	2.8±0.4	1.3±0.3
SpCAF-1-ED*	1.0±0.1	2.3±0.1
SpCAF-1-PIP*	0.7±0.1	2.7±0.3