Table 2. Interactions parameter with SpPCNA measured by isothermal microcalorimetry (ITC).
| Ligand | Kd (μM) | ΔG (kCal.M–1) | N* | ΔH (kCal.M–1) | -TΔS (kCal.M–1) |
|---|---|---|---|---|---|
| Pcf1_PIP | 7.1±1.3 | –6.9±0.1 | 0.97±0.08 | –2.9±0.2 | –0.39±0.3 |
| Pcf1_PIP* | Not detected | ND | ND | ND | ND |
| Pcf1_KER-PIP | 0.7±0.2 | –8.2±0.2 | 0.64±0.04 | +2.9 ± 0.6 | –11.2±0.8 |
| Pcf1_KER*-PIP | 7.1±1.5 | –6.9±1.2 | 0.7±0.2 | +1.0 ± 0.5 | –7.9±0.7 |
| Pcf1_KER-PIP* | Not detected | ND | ND | ND | ND |
| Cdc27_PIP | 3.5±0.3 | –7.3±0.1 | 0.9±0.1 | –4.8±0.02 | –2.4±0.1 |
*The stoichiometry (N) is calculate as a molar ratio of monomeric PCNA.