Appendix 10—figure 2. Potentials of mean force (PMFs) along the pore axis of Nsp1-coated pores with various diameters and grafting densities using a probe radius of r_probe = 34 Å (BSA) derived from void analysis.

The height of the PMF barrier, ${\displaystyle \mathrm{\Delta }E}$ given on the right of the plots, is obtained by averaging the PMF between –25 nm ≤ z ≤ 25 nm (gray region). The location of the peaks in the PMF curves is at the same ${\displaystyle z}$-coordinates as the anchoring points of the Nsp1 proteins.