Appendix 11—figure 1. Rate changes due to presence of Kap95.

(A) Rate change of Kap95 vs. pore diameter. Event rate of Kap95 at 1000 nM (k_Kap95,1000) divided by the Kap95 event rate at 100 nM (k_Kap95,1000). At high Kap95 concentrations, there is an increase in normalized event rate for both open and Nsp1 pores. It is largest for small Nsp1 pores. (B) The averages indicated in the plots by horizontal lines given with standard deviation estimated from fitting horizontal lines. (C, D) Influence of BSA concentration on Kap95 event rate. When dividing the Kap95 event rate with 250 nM of BSA being present ${\displaystyle k_{\mathrm{K}\mathrm{a}\mathrm{p}95,\mathrm{B}250}}$ by the Kap95 event rate with 0 nM of BSA being present ${\displaystyle k_{\mathrm{K}\mathrm{a}\mathrm{p}95,\mathrm{B}0}}$ (C), this shows that the Kap95 event rate is barely influenced by the presence of BSA, as expected. The same holds for the Kap95 event rate change with 500 nM of BSA to 0 nM being present. (E, F) Selectivity vs. pore diameter for different Kap95 concentrations. When switching from 100 nM of Kap95 to 1000 nM of Kap95, the selectivity of small Nsp1 pores increases by a factor of 3, whereas the selectivity of large-pore decreases.