Appendix 13—figure 1. Molecular brightness for different time traces.

(A, B) Molecular brightness per time trace versus pore diameter on Nsp1 pores. We see two populations for Kap95, labeled in purple and yellow. The yellow datasets show a decreased molecular brightness and therefore these datasets were removed from further analysis. (C, D) Comparison of molecular brightness between open pores (cyan), Nsp1 pores (purple), and free diffusion (black horizontal line). For Kap95, we found barely any difference of the data distribution. For BSA there, we observed a decrease in molecular brightness for small pores of Nsp1. This can be explained by the lower event rate in these pores. Different markers show different experiments.