Appendix 13—figure 2. Effect of time trace removal.

(A, B) Event rates of discarded pores of Nsp1. For completeness, we show the event rates of pores that were not further considered in the analysis (yellow) due to their lower molecular brightness, as shown in the previous figure. As expected, these pores show a much decreased normalized event rate both for Kap95 and BSA compared to the Nsp1 pores that were kept in the analysis (purple). (C, D) Zoom-in of (A, B). (E) Selectivity of discarded pores. The selectivity of discarded pores (yellow) deviates from the selectivity of the kept pores (purple). This can be explained when taking into account that a changed molecular brightness influences the event detection in each channel differently.