Appendix 5—figure 1. Free diffusion fits to experimental data.

(A, B) Normalized and averaged event rate vs pore diameter as in Figure 4. Solid lines are fits of a diffusion model as described in this section. A single fit for BSA through Nsp1 would deviate both for small- and large-pore diameters. Therefore, the dataset was split into two regimes: below 50 nm and above 60 nm in order to fit the data. This threshold was inspired by the results of the coarse-grained molecular dynamics (CGMD) simulations. The dashed line shows a fit of the shifted quadratic function to the BSA data through Nsp1 pores as described in the main text. (C, D) Zoom-ins of (A, B). (E) Selectivity of open pores, predicted by the diffusion model. The value is pore size dependent because of the different sizes of Kap95 and BSA.