Appendix 6—figure 2. Simple quadratic fit to experimental data.

(A, B) Normalized and averaged event rate vs. pore diameter as in Figure 4. Solid lines are fits of a quadratic function as given in Equation 16. The fit for BSA through Nsp1 deviates both for small- and large-pore diameters. Therefore, a shift parameter was introduced as given in Equation 17 to fit the data (dashed line).(C, D) Zoom-ins of (A, B). (E) Open pore event rates obtained from the quadratic fit divided by Nsp1 pore event rates obtained from the quadratic fit vs. pore diameter. While there is barely any decrease of the event rate for Kap95 when Nsp1 is present, BSA experiences an approximately tenfold decrease for small pores of 35 nm diameter. This ratio increases with pore diameter and approaches a value of 1 in the limit to very large pores.