Figure 4. Dependence of translocation rates and selectivity on pore diameter.

(A–D) Concentration-normalized event rate of Kap95 (A) and BSA (B) as a function of pore diameter for open pores (cyan squares) and Nsp1-coated pores (purple circles). While the normalized event rate for Kap95 did not change significantly between open and Nsp1-coated pores, a clear reduction was observed for BSA, which was most pronounced at small pore sizes. Solid lines are fits to a quadratic function given in Equation 2. To model the size dependence of BSA translocations through Nsp1 pores, an offset was introduced that shifts the onset of the quadratic curve to higher diameters (dashed line, Equation 3). (C, D) Zoom-ins of the indicated regions in (A ,B). In (A–D), the error bars represent the standard error of the mean of the normalized event rates obtained at different protein concentrations. (E) Apparent selectivity versus pore diameter. The data show that selectivity was lost for Nsp1-coated pores with increasing diameter. The average selectivity for open pores of 0.70 ± 0.02 is shown by the dashed cyan line. Error bars indicate the propagated error from the normalized event rates shown in (A–D).