Table 3. List of filters and lasers used for in situ sequencing.
| Filters | ||
|---|---|---|
| Main dichroic | Filter names | |
| D1 | FF421/491/567/659/776-Di01 (Semrock) | |
| D2 | ZT405/514/635rpc | |
| D3 | FF421/491/572-Di01−25x36(Semrock) | |
| Emission filters | ||
| E1 | FF01-441/511/593/684/817(Semrock) | |
| E2 | FF01-565/24(Semrock) | |
| E4 | FF01-676/29(Semrock) | |
| E5 | FF01-775/140(Semrock) | |
| E7 | 69,401 m | |
| E8 | ZET532/640 m | |
| Imaging settings | ||
| Sequencing cycles | ||
| Channel | Filter combinations | laser |
| G | D2/E2 | 520 |
| T | D1/E1 | 546 |
| A | D2/E4 | 638 |
| C | D2/E5 | 638 |
| DIC | D2/E5 | DIA |
| Hybridization cycle | ||
| GFP | D3/E7 | 477 |
| YFP | D2/E2 | 520 |
| TxRed | D3/E7 | 546 |
| Cy5 | D2/E8 | 638 |
| DAPI | D1/E7 | 405 |
| DIC | D3/E7 | DIA |