Table 1. Nanobodies (Nbs) binding.
| Titrant | Titrate condition | Kd(µM) | Number of tests | Fold of affinity change | p-Value* |
|---|---|---|---|---|---|
| Nb725_4 | MelBSt/Na+ | 3.64 ± 0.62† | 7 | ||
| MelBSt/Na+/melibiose | 11.81 ± 0.72 | 6 | -3.24 | <0.01 | |
| MelBSt/Na+/α- †NPG |
14.63 ± 1.27 | 2 | -4.02 | <0.01 | |
| MelBSt/Na+/EIIAGlc | 2.14 ± 0.11 | 2 | +1.70 | >0.05 | |
| NabFab | 52.53 ± 13.03‡ | 2 | |||
| Nb725 | MelBSt/Na+ | 1.58 ± 0.42 | 6 | ||
| MelBSt/Na+/melibiose | 8.88 ± 1.24 | 5 | -5.62 | <0.01 | |
| MelBSt/Na+/α-NPG | 12.58 ± 0.60 | 2 | -7.96 | <0.01 | |
| MelBSt/Na+/EIIAGlc | 1.18 ± 0.12 | 2 | +1.34 | >0.05 | |
| NabFab | 36.02 | 1 | |||
| Anti-Fab Nb | NabFab | 112.90 ± 16.33‡ | 2 |
*
Unpaired t-test.
†
Mean ± SEM.
‡
nM.