Table 2. Nanobody (Nb) effects on MelBSt binding to sugar, Na+, and EIIAGlc.
| Titrant | Titrate condition | Kd(µM) | Number of tests | Fold of affinity change | p-Value* | Reference |
|---|---|---|---|---|---|---|
| Melibiose | MelBSt/Na+ | 1430 ± 30.0† | 5 | / | ||
| MelBSt/Na+/Nb725_4 | /‡ | 3 | ||||
| MelBSt/Na+/Nb725 | / | 3 | ||||
| MelBSt/Na+/EIIAGlc | / | 3 | Hariharan et al., 2015 | |||
| α-NPG | MelBSt/Na+ | 16.46 ± 0.21 | 5 | / | ||
| MelBSt/Na+/Nb725_4 | 531.55 ± 19.25 | 2 | -32.29 | <0.01 | ||
| MelBSt/Na+/Nb725 | 353.55 ± 37.25 | 2 | -21.48 | <0.01 | ||
| MelBSt/Na+/EIIAGlc | 76.13 ± 4.52 | 2 | -4.63 | <0.01 | Hariharan et al., 2015 | |
| Na+ | MelBSt | 261.77 ± 49.15 | 4 | / | ||
| MelBSt/Nb725_4 | 345.04 ± 9.92 | 2 | -1.32 | >0.05 | ||
| MelBSt/Nb725 | 182.50 ± 11.30 | 2 | +1.43 | >0.05 | ||
| MelBSt/EIIAGlc | 253.50 ± 11.00 | 2 | +1.03 | >0.05 | Katsube et al., 2023 | |
| EIIAGlc | MelBSt/Na+ | 3.76 ± 0.29 | 5 | / | ||
| MelBSt/Na+/Nb725_4 | 4.32 ± 0.64 | 4 | -1.15 | >0.05 | ||
| MelBSt/Na+/Nb725 | 1.94 ± 0.10 | 4 | +1.94 | <0.01 |
*
Unpaired t-test.
†
Mean ± SEM.
‡
Not detectable.