Table 4. HDX reaction and labeling details.

Samples measured	WT MelBSt	WT MelBSt complexed with Nb725m
HX reaction buffer	25 mM Tris-HCl, pD 7.5, 150 mM NaCl, 10% Glycerol, and 0.01% DDM
Reaction temperature (°C)	20
HX time course (s)	0, 30, 300, 3000
Number of peptides	153
Sequence coverage by labeling	86%
Mean peptide length	9.3
Average redundancy	2.9
Replicates (technical)	3
|ΔD| (Da)	0.3071
Back exchange rate	Not appliable