| Cell line (Homo sapiens) |
HEK293T |
ATCC |
HEK293T |
Cat# CRL-3216; RRID:CVCL_0063
|
| Cell line (Mus musculus) |
N2a |
ATCC |
N2a |
Cat# CCL-131; RRID:CVCL_0470
|
| Cell line (M. musculus) |
C2C12 |
ATCC |
C2C12 |
Cat# CRL-1772 |
| Transfected construct (H. sapiens) |
TREK-1 |
PMID:18004376
|
Dr. Steven Long (Sloan Kettering) |
|
| Transfected construct (M. musculus) |
mPLD2 |
PMID:9867870
|
Dr. Michael Frohman (Stony Brook) |
|
| Transfected construct (M. musculus) |
xPLD2 |
PMID:9867870
|
Dr. Michael Frohman (Stony Brook) |
Catalytically dead PLD2 |
| Cell line (Drosophila melanogaster) |
BG2-c2 |
Drosophila Genomics Resource Center |
DGRC Stock 53; https://dgrc.bio.indiana.edu//stock/53; RRID:CVCL_Z719
|
|
| Strain, strain background (Drosophila melanogaster) |
PLDnull |
PMID:27848911
|
|
|
| strain, strain background (D. melanogaster) |
PLD-KD |
Vienna Drosophila Resource Center |
Stock#: v106137 |
|
| Biological sample (M. musculus) |
SREBP2-KO |
PMID:34385305
|
Heather Ferris (University of Virginia) |
Brain slices from SREBP2-KO animals |
| Antibody |
Anti-TREK-1 (rabbit, polyclonal) |
Santa Cruz |
Cat# sc-50412; RRID:AB_2131048; |
1:100 dilution |
| Antibody |
Anti-TREK-1 (mouse, monoclonal) |
Santa Cruz |
Cat# sc-398449 |
1:100 dilution |
| Antibody |
Anti-PIP2 (mouse, monoclonal) |
Echelon Biosciences |
Cat# Z-P045, RRID:AB_427225
|
1:100 dilution |
| Antibody |
Anti-rabbit Alexa 647 (goat, polyclonal) |
Thermo Fisher Scientific |
Cat# A-21244, RRID:AB_2535812
|
1:1000 dilution |
| Antibody |
Anti-mouse Alexa 647 (goat, polyclonal) |
Thermo Fisher Scientific |
Cat# A-21235, RRID:AB_2535804
|
1:1000 dilution |
| Antibody |
Anti-mouse cy3B (donkey, polyclonal) |
PMID:27976674
|
Cat# NC9812063 |
1:1000 dilution |
| Chemical compound, drug |
CTxB |
Thermo Fisher Scientific |
Cat# C34778
|
|
| Peptide, recombinant protein |
ApoE3 |
BioLegend, USA |
Cat# 786802 |
|
| Chemical compound, drug |
Atto 647 |
Sigma-Aldrich |
18373-1MG-F |
|
| Chemical compound, drug |
Cholesterol oxidase |
Sigma-Aldrich |
C8649-250UN |
|
| Chemical compound, drug |
Amplex red |
Cayman Chemical |
Cat# 10010469 |
|
| Peptide, recombinant protein |
Horseradish peroxidase |
VWR |
516531-5KU |
|
| Peptide, recombinant protein |
Choline oxidase |
VWR |
Cat# 15349250 |
|
| Chemical compound, drug |
C8-PC |
Avanti Lipids |
Cat# 850315P |
|
| Peptide, recombinant protein |
Glucose oxidase |
Sigma-Aldrich |
Cat# G2133 |
|
| Peptide, recombinant protein |
Catalase |
Sigma-Aldrich |
Cat# C40 |
|
| Chemical compound, drug |
Maleimide cy3B |
GE-Health |
Cat# PA63131 |
|
