Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Haemophilus influenzae Rd KW20) | SiaP; SiaQM | UniProt | P44542, P44543 | |
| Strain, strain background (Escherichia coli) | BL21 (DE3); TOP10 | Invitrogen | Chemically competent cells | |
| Antibody | Anti-Xpress IgG (mouse monoclonal) | Invitrogen | WB (1:2500) | |
| Antibody | Anti-mouse IgG (rabbit) | Sigma-Aldrich | WB (1:15000) | |
| Recombinant DNA reagent | pET22b(+) (plasmid) |
GenScript | Provides pelB leader sequence | |
| Recombinant DNA reagent | pBAD/HisA (plasmid) |
Thermo Fisher Scientific | ||
| Peptide, recombinant protein | cNW11 | Now Scientific | Covalent nanodisc protein |