Figure 4. Early life adversity-associated CpG sites are not enriched for mSTARR-seq regulatory activity.

Log₂-tranformed odds ratios from Fisher’s Exact Tests for enrichment relative to the background set of sites on each array platform, for 27 studies of early life adversity-DNA methylation level correlations (see Supplementary file 20 for full FET results). Whiskers show standard error. Only Cao-Lei et al., 2014 shows significant enrichment for regulatory activity (log₂(OR) [95% CI]=1.343 [0.715, 1.909], p=3.39 x 10^–5), but these sites are not more likely to exhibit methylation-dependent activity than expected by chance (log₂(OR) [95% CI]=0.884 [-0.338, 2.130], p=0.16). For details on the source tissue and measures of ELA, see Figure 4—figure supplement 1.

Figure 4—figure supplement 1. Summary of early-life adversity (ELA) studies.

Colored rectangles indicate ranges of age at adversity. Circles represent mean ages at sample collection and are colored according to the tissue type used to measure methylation levels. Ranges and standard errors of ages at sample collection, when reported, are indicated by gray rectangles and horizontal black solid lines, respectively. See Supplementary file 20 for results of Fisher’s exact tests assessing enrichment of ELA-associated CpGs for regulatory activity.