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. 2024 Jan 5;12:RP87965. doi: 10.7554/eLife.87965

Figure 4. Dag1 is required for CCK+/CB1R+ IN axon targeting during early postnatal development.

Figure 4.

(A) Immunostaining for CB1R+ axon terminals (green) in the hippocampus of Emx1Cre:Dag1 controls (left) and cKOs (right) at ages P5-P30. Nuclear marker Hoechst is shown in magenta. White arrowheads indicate migration errors in dentate granule cells Emx1Cre:Dag1 cKO mice. Scale bar = 500 μm. (B) Higher magnification images of CB1R+ axon terminals in CA1 of Emx1Cre:Dag1 controls (left) and cKOs (right) at ages P5-P30. Scale bar = 100 μm. (C) Quantification of CB1R fluorescence intensity profile in CA1. Shaded regions of intensity profile illustrate ± SEM. Gray region highlights SP. See Supplementary file 1 for Ns. Significance: *=p < 0.05, **=p < 0.01, ***=p < 0.001, NS = p ≥ 0.05. A.U., arbitrary units; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum.

Figure 4—source data 1. Raw data for quantification in Figure 4C.