FIG. 6.
Schematic representation of defined DNA fragments from BDCRB-resistant virus used in marker transfer experiments. (Line 1a) Genome structure of HCMV strain AD169. Boxes marked TRL and TRS are terminal repeat elements, and boxes marked IRL and IRS are internal repeat elements. Directly beneath the UL region are indicated the approximate locations of ORFs (by number). (Line 1b) Schematic representation showing location and overlap of cosmids from a library generated from 1038rB 8-3-3 DNA. Thick bars represent cosmids which transferred resistance, solid lines represent cosmids which did not transfer resistance, and a dashed line represents a cosmid which was not tested. (Line 2a) EcoRI and HindIII restriction sites within the pCU11-pCU32 overlap shown in line 1b. (Line 2b) Schematic colinear representation of two fragments isolated from pCU11 (HindIII P and EcoRI C) which transferred resistance. (Line 3a) Schematic representation of the 6,320-bp pCU11-pCU32 overlap fragment, with restriction sites indicated by vertical lines. Directly below this line is a schematic colinear representation in which the horizontal arrows indicate the lengths and orientations of the indicated ORFs. (Line 3b) Schematic colinear representation of restriction fragments isolated from the 6,320-bp fragment used in marker transfers. The fragments are named according to the restriction sites at their ends. Thick bars represent fragments which transferred resistance in marker transfer experiments, while thin bars represent fragments which were unable to transfer resistance. (Line 3c) Schematic representation of PCR products amplified from BDCRB-resistant virus DNA and used for genetic transfers and sequencing. A solid bar represents the fragment that transferred BDCRB resistance. (Line 4) Schematic representation of the 394-bp region which conferred resistance in marker transfer experiments. Below line 4 is shown a schematic colinear representation in which the horizontal arrows indicate the lengths and orientations of the indicated ORFs. Restriction endonuclease abbreviations: E, EcoRI; H, HindIII; K, KpnI; N, NotI; Ps, PstI; Pv, PvuI; X, XbaI.