TABLE 1.
Ratios of UL-US junction and end fragment UL-W concentration to internal fragment UL-O concentration in the presence or absence of BDCRB during synthesis and maturation of HCMV DNAa
| Fragment | Ratio of fragment to UL-O fragment
|
||||||
|---|---|---|---|---|---|---|---|
| AD169 virion DNAb | 72 h
|
96 h
|
120 h
|
||||
| − | + | − | + | − | + | ||
| Junction | 1.05 | 1.21 | 1.18 | 1.32 | 1.22 | 0.97 | 1.27 |
| UL-O | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| UL-W | 0.12 | 0 | 0 | 0.02 | 0 | 0.14 | 0.015 |
a
Ratios were calculated from Southern analyses run as described in the legend to Fig. 4 in either the presence (+) or absence (−) of 25 μM BDCRB. All values were normalized to the amount of the EcoRI O fragment generated. Samples were collected at the postinfection times shown.
b
AD169 virion DNA was isolated and digested in side-by-side experiments, and fragment ratios were calculated as a control for linear DNA.