TABLE 3.
Plating efficiencies for genetic mapping experiments
| Expta | Transfected DNAb | Plating efficiency (%)c |
|---|---|---|
| 1 | None | <0.02 |
| pCU11 | 2.7 | |
| pCU27 | <0.02 | |
| pCU32 | 0.41 | |
| 2 | None | <0.01 |
| KE | 0.14 | |
| PsPs | 0.12 | |
| PvPv | 0.54 | |
| 3 | None | <0.01 |
| PCR-B12 | 0.10 | |
| PCR-B34 | <0.01 | |
| 4 | None | <0.01 |
| PCR-A12d | 0.34 | |
| PCR-C12d | 0.36 |
Experiments 1 through 3 were run with cosmids or different DNA fragments from strain 1038rB 8-3-3. Four other cosmids tested in experiment 1 which are not shown in this table had plating efficiencies of <0.02%. Similarly, restriction fragments tested in experiment 2 which are not shown had plating efficiencies of <0.01%. Experiment 4 was a mapping experiment run with PCR fragments from 1038rA 5-2-3 or 1038rC 4-3-2.
None, negative control in which AD169 was precipitated without additional fragments. Italicized designations represent restriction sites at the ends of the fragments and correspond to abbreviations defined in the legend to Fig. 6.
Plating efficiency is the percentage of plated marker transfer progeny which formed plaques in the presence of 2 μM BDCRB.
PCR amplification products from 1038rA 5-2-3 and 1038rC 4-3-2 (i.e., PCR-A12 and PCR-C12, respectively) were generated with PCR primers as described in Materials and Methods and transfected into cells.