Key resource table : RNAseq resource tables
| Reagent or resource | Source | Catalogue # |
|---|---|---|
| Antibodies | ||
| Donkey anti-rabbit IgG, PE conjugated | Jackson ImmunoReserch | 711-116-152 |
| Donkey anti-rabbit IgG, AlexaFluor 647 | Thermo Fisher Scientific | A32795 |
| Goat anti-mouse, AlexaFluor 647 conjugated | Abcam | A21235 |
| Goat anti-mouse, Alexa 488 conjugated | Thermo Fisher Scientific | A11029 |
| Goat anti-mouse, APC conjugated | BD Biosciences | 550826 |
| Moues anti-PDGFRα, PE conjugated | BD Biosciences | 556002 |
| Mouse anti-CD235a, APC conjugated | BD Biosciences | 551336 |
| Mouse anti-CD56, APC conjugated | BD Biosciences | 555518 |
| Mouse anti-human cTnT | Thermo Fisher Scientific | MS-295-P1 |
| Mouse anti-cardiac troponin T isoform Ab1 | Thermo Fisher Scientific | MA512960 |
| Rabbit anti-human MLC2v | Abcam | ab79935 |
| Phalloidin 488 | Thermo Fisher Scientific | A12379 |
| Mouse anti-α-actinin | Abcam | Ab9465 |
| Biological samples | ||
| Human cardiac fibroblasts | PromoCell | C-12375 |
| Commercial assays | ||
| iScript™ reverse transcription supermix | Bio-Rad | 1708841 |
| SuperScript™ III reverse transcriptase | Invitrogen | 18080085 |
| QuantiFast SYBR green PCR kit | Qiagen | 204057 |
| RNAqueous™-Micro total RNA isolation kit | Ambion | AM1931 |
| Chemicals, peptides and proteins | ||
| 1x Medium 199 | Sigma | M0650 |
| Activin A | Bio-Techne | 338-AC-01M/CF |
| Ascorbic acid | Sigma | A4544-100G |
| Basic fibroblast growth factor | Bio-Techne | 233-FB |
| Bone morphogenic protein 4 | Bio-Techne | 314-BP |
| Bovine serum albumin | Sigma | A1470 |
| Collagenase Type II | Worthington | LS004176 |
| DAPI | ThermoFisher | 62248 |
| Dofetilide | Sigma | PZ0016 |
| Dulbecco’s modified eagle media/F12 | Cellgro | MT10092CV |
| Fetal bovine serum | Fisher/HyClone | SH3039603Q |
| Flecainide | Sigma | F6777 |
| Fluo-4 NW | Molecular Probes | F36205 |
| Fura-2 AM | Molecular Probes | F14201 |
| Gelatin | Sigma | G1890 |
| Glutamax | Gibco | 35050061 |
| Growth factor reduced Matrigel | Fisher/Corning | 354230 |
| Iscove’s modified Dulbecco’s media | Gibco | 12200069 |
| IWP2 | Bio-Techne | 3533/10 |
| KnockOut™ serum replacement | Gibco | 10828028 |
| L-ascorbic acid | Sigma | A8960 |
| L-glutamine | Gibco | 25030081 |
| Monothioglycerol | Sigma | M6145-25ML |
| Mounting media with DAPI | Abcam | ab104139 |
| Non-essential amino acids | ThermoFisher | 11140-050 |
| Penicillin/streptomycin | Gibco | 15070063 |
| Pluronic F127 | Sigma | P2443 |
| Poly(2-hydroxyethyl methacrylate) | Sigma | P3932-25G |
| Polyurethane 2-part adhesive | GS Polymers, Inc. | GSP 1552-2 |
| Collagen I, Rat tail, High concentration | Corning | 354249 |
| Rho-associated protein kinase inhibitor Y-27632 | Bio-Techne | 1254/50 |
| (S)-(−)-Blebbistatin | Toronto Research Chemicals | B592500 |
| StemPro-34 media | Gibco | 10639011 |
| Transferrin | Sigma/Roche | 10652202001 |
| TrypLE™ Express (1x), Phenol Red | Gibco | 12605010 |
| Vascular endothelial growth factor | Bio-Techne | 293-VE |
| β-Mercaptoethanol | Gibco | 21985-023 |
| Hyaluronidase | Sigma | H3506 |
| Dispase | Stem Cell Technologies | 07913 |
| HBSS | Invitrogen | 14175-103 |
| DNase I | Calbiochem | 260913 |
| Software | ||
| BD FACSDiva™ | BD Biosciences | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software |
| BrainVision software | SciMedia | http://www.scimedia.com |
| CellSens software | Olympus | https://www.olympus-lifescience.com/en/software/cellsens/ |
| FlowJo | Tree Star | https://www.flowjo.com |
| GraphPad Prism 7.0 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| IDL | Harris Geospatial | https://www.harrisgeospatial.com/Software-Technology/IDL |
| Matlab | Mathworks | https://www.mathworks.com/products/matlab.html?s_tid=hp_products_matlab |
| MasterCycler ep Realplex software | Eppendorf | https://www.qiagen.com/ca/resources/resourcedetail?id=adcdeee3-98c7-43e8-987f-a84e4cfe01d2&lang=en |
| pClamp 8, 9 and 10 | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite |
| Zen software | Zeiss | https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html |