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. 2022 May 25;8(21):eabm0972. doi: 10.1126/sciadv.abm0972

Fig. 4. A population of gonadal cells expressing both GATA4 and PAX8 is present from E10.5 to E11.0 along the genital ridge next to the mesonephros.

Fig. 4.

Whole-mount IF of XY (A, C, and E) and XX (B, D, and F) genital ridges at E10.5 (A), E11.0 (B), E11.5 (C and D), and E12.5 (E and F). Boxes in merged images indicate regions shown as isolated channels on right. The dotted line corresponds to the border that separates the GATA4+ cells of the genital ridge (G) from the mesonephric cells (M). LAMB1 is expressed in the basement membrane throughout the CE and mesonephric tubules. SOX9 overlaps with a subset of PAX8+/GATA4+ cells in XY and XX tissues at E11.5. NR2F2 is a marker of gonad interstitial cells and mesonephric mesenchyme. Arrowhead indicates SOX9+/PAX8+ cell within the XY gonad at E11.0. Arrow indicates PAX8+/GATA4− cells adjacent in the region of the presumptive rete that are not within mesonephric tubules. Scale bars, 50 μm.