FIG. 7.

Reactivities of substituted antigenic peptide A15 with site A-specific MAbs. Competitive ELISA were performed as described in Materials and Methods. In each panel the MAb used is indicated at the top. The residues of peptide A15 are listed in the left column, and the amino acids used for single-site substitution are listed in the top row. Relative IC₅₀s are indicated as black (IC₅₀ > 100), heavily striped (IC₅₀ = 30 to 100), lightly striped (IC₅₀ = 5 to 30), or empty (IC₅₀ < 5) boxes. The last two columns indicate in symbolic and numerical forms the average IC₅₀s for all tested substitutions of a given residue. Primary data for IC₅₀ determinations will be provided upon request. The origin of each MAb and the procedure used for synthesis of replaced peptides are given in Materials and Methods.