Table 2.
The quality indicators of 6 platelet pool samples.
| Sample name | CA1 | CA2 | CA3 | PBS1 | PBS2 | PBS3 |
|---|---|---|---|---|---|---|
| RNA concentrationa | 119 pg/μL | 73 pg/μL | 183 pg/μL | 232 pg/μL | 123 pg/μL | 242 pg/μL |
| RIN valuea | 8.1 | 8.2 | 6.0 | 7.3 | 7.5 | 7.2 |
| Raw readsb | 47,768,682 | 47,994,714 | 45,691,272 | 47,923,640 | 45,550,924 | 46,829,908 |
| Raw basesb | 7.17 G | 7.20 G | 6.85 G | 7.19 G | 6.83 G | 7.02 G |
| Error rateb, % | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 |
| Q20b, % | 97.36 | 97.68 | 97.45 | 97.56 | 97.31 | 97.47 |
| Q30b, % | 93.19 | 93.78 | 93.47 | 93.61 | 93.11 | 93.39 |
| GCb, % | 50.44 | 49.79 | 49.52 | 49.37 | 48.24 | 48.70 |
| Total mapc, % | 95.05 | 93.16 | 92.95 | 96.21 | 83.91 | 96.02 |
| Multimapc, % | 11.58 | 6.17 | 6.06 | 7.50 | 5.48 | 6.65 |
| Exonc, % | 96.25 | 95.36 | 95.73 | 96.09 | 95.94 | 96.11 |
| Intronc, % | 2.14 | 2.51 | 2.40 | 2.21 | 2.37 | 2.24 |
| Intergenicc, % | 1.61 | 2.13 | 1.86 | 1.70 | 1.69 | 1.65 |
CA, caffeic acid; PBS, phosphate-buffered saline, RIN, RNA integrity number.
a
The indicators of RNA quality and each RNA sample were diluted 100- to 200-fold for measurement.
b
The quality indicators of sequencing reads.
c
The reference sequence alignment results.