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. 2024 Feb 15;43(6):956–992. doi: 10.1038/s44318-024-00049-w

Figure EV1. let-381 motifs are required for endogenous GLR gene expression and let-381 autoregulation in GLR glia.

Figure EV1

(A) let-381 motif identified in this study. (B) Motif of the let-381 ortholog foxf from (Peterson et al, 1997). (C) let-381 motif from (Narasimhan et al, 2015). Similarities between the three motifs are apparent. (D) Locations (distances from start codons) of let-381 motifs of genes whose expression in GLR is downregulated in let-381 mutants (either GLR-specific let-381 RNAi and/or the let-381 autoregulatory allele). (E) Minimal promoter hlh-1prom1 was one of the promoters used in MEME to identify common motifs present in GLR glia genes. The let-381 motif identified by MEME is highlighted in dark red. A let-381 motif with slightly altered sequence (light red) was identified manually later and is required, together with the first motif, to control hlh-1 expression in GLR glia. (F) Schematics showing details on endogenous gfp-based tags, location of let-381 motifs and their mutation for nep-2, pll-1, hlh-1 and inx-18 genes. Red bars represent let-381 motifs. Distance from ATG is indicated above each motif. Nucleotide changes for each motif mutation is shown below the motifs.