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. 2024 Feb 15;43(6):956–992. doi: 10.1038/s44318-024-00049-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV2 — (A) Schematic showing the location of the let-381 motif (red bar) in the let-381 promoter region and region deleted in the let-381(ns1026) mutation. (B) Conservation of the let-381 autoregulatory motif sequence (red box) is shown among five nematode species. Asterisks indicate conserved nucleotides. (C–G) Effect of let-381 autoregulatory motif deletion, let-381(ns1026), on expression of (C) gly-18, (D) hot-5, (E) lgc-55, (F) gbb-2, and (G) snf-11 in GLR glia. Bar graphs show quantifications of gene expression at the L4 stage. For (C) animal images showing gene expression at L1 and L4 stages in wild-type and mutant backgrounds are shown on the left. Dashed red circles outline expression in GLR glia. Data information: Anterior is left, dorsal is up and scale bars are 10 μm for all animal images.